4ZGP

Structure of Cdc123 from Schizosaccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-28
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	86.483, 91.436, 86.006
Unit cell angles	90.00, 91.24, 90.00

Refinement procedure

Resolution	43.200 - 1.850
R-factor	0.1899
Rwork	0.188
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zgo
RMSD bond length	0.008
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	1.960
High resolution limit [Å]	1.850	1.850
Rmerge		0.476
Number of reflections	56189
<I/σ(I)>	19	2.6
Completeness [%]	98.1	96.5
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	297	12% PEG3350, 4% tacsimate