4ZGO
Structure of C-terminally truncated Cdc123 from Schizosaccharomyces pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.600, 91.710, 86.240 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 45.800 - 2.063 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | low-resolution model from SAD experiments |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.093 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.190 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.908 | |
| Number of reflections | 41180 | |
| <I/σ(I)> | 15.7 | 1.3 |
| Completeness [%] | 99.0 | 94.3 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 12% PEG3350, 4% tacsimate |
