4ZGN
Structure Cdc123 complexed with the C-terminal domain of eIF2gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.260, 116.640, 132.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.900 |
| R-factor | 0.1983 |
| Rwork | 0.195 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZGO and 2AHO |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.875 | |
| Number of reflections | 13237 | |
| <I/σ(I)> | 15.8 | 2.2 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 25%PEG3350, 0.2MLiSO4, 0.1M TrispH8.0 |
