4ZGI

Structure of Truncated Human TIFA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2014-10-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791, 0.9640
Spacegroup name	P 32 2 1
Unit cell lengths	39.009, 39.009, 178.459
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.829 - 2.701
R-factor	0.23
Rwork	0.226
R-free	0.26440
RMSD bond length	0.012
RMSD bond angle	1.165
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.800
High resolution limit [Å]	2.700	5.770	2.700
Rmerge	0.064	0.025	0.548
Rmeas	0.068	0.026	0.596
Rpim	0.023	0.009	0.224
Total number of observations	41786
Number of reflections	4730
<I/σ(I)>	9.8
Completeness [%]	98.3	96.9	96.6
Redundancy	8.8	8	6.4
CC(1/2)		0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	80mM LiCl, 0.2M trimethylamine N-oxide, 15% PEG 3350, 100mM HEPES