4ZGG
Crystal structure of a DJ-1 (PARK7) from Homo sapiens at 1.23 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.002, 75.002, 74.897 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.796 - 1.230 |
| R-factor | 0.1172 |
| Rwork | 0.116 |
| R-free | 0.13390 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.697 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.796 | 29.796 | 1.260 |
| High resolution limit [Å] | 1.230 | 5.500 | 1.230 |
| Rmerge | 0.080 | 0.804 | |
| Rmeas | 0.124 | 0.087 | 0.913 |
| Rpim | 0.046 | 0.033 | 0.422 |
| Total number of observations | 479140 | 5817 | 18482 |
| Number of reflections | 69760 | ||
| <I/σ(I)> | 8.1 | 17.1 | 1.7 |
| Completeness [%] | 98.3 | 98.9 | 86.1 |
| Redundancy | 6.9 | 6.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 5.00% polyethylene glycol 6000, 0.1M TRIS pH 8.0 |
