4ZG3
In-vacuum long-wavelength crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 45 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.378 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.842, 57.842, 150.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.986 - 1.200 |
| R-factor | 0.1477 |
| Rwork | 0.147 |
| R-free | 0.16750 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.370 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.037 | 0.760 |
| Number of reflections | 138112 | |
| <I/σ(I)> | 26.7 | 2.2 |
| Completeness [%] | 89.9 | 79.9 |
| Redundancy | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 292 | 0.5 M K/Na Tartrate, 0.05 M ADA, ph6.8, 20 % glycerol |
