4ZG0
Crystal structure of Mouse Syndesmos protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2007-04-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.318, 68.701, 66.191 |
| Unit cell angles | 90.00, 103.93, 90.00 |
Refinement procedure
| Resolution | 37.044 - 2.006 |
| R-factor | 0.1815 |
| Rwork | 0.178 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cou |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.155 |
| Number of reflections | 26347 | |
| <I/σ(I)> | 34.79 | |
| Completeness [%] | 96.5 | 89.3 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.5 | 290 | 0.2M Li sulfate, 0.1M Bis-Tris, 25%(m/w) PEG3350 |
