4ZFW
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd) in complex with galactose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 142.300, 137.070, 44.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.540 - 2.540 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8s |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.666 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.300 | 2.670 |
| High resolution limit [Å] | 2.533 | 2.530 |
| Rmerge | 0.800 | |
| Number of reflections | 29954 | |
| <I/σ(I)> | 11.7 | 2.4 |
| Completeness [%] | 99.0 | 93.4 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1M HEPES, 20% w/v PEG 10000, glycerol |
