4ZFS
Phototoxic Fluorescent Protein KillerOrange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-15 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 128.930, 202.064, 116.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.850 - 2.010 |
| R-factor | 0.1923 |
| Rwork | 0.192 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zbl |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.124 | 0.081 | 0.435 |
| Rmeas | 0.127 | 0.083 | 0.450 |
| Rpim | 0.030 | 0.020 | 0.113 |
| Total number of observations | 1724142 | ||
| Number of reflections | 101443 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 17 | 17.4 | 14.7 |
| CC(1/2) | 0.998 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.02M Na acetate, 1% gamma-PGA (Na+ form, LM), 3% PEG 4000 |
