4ZFR
Catalytic domain of Sst2 F403A mutant bound to ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.139, 58.174, 56.442 |
Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
Resolution | 53.270 - 1.720 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k1r |
RMSD bond length | 0.018 |
RMSD bond angle | 1.896 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.270 | 1.750 |
High resolution limit [Å] | 1.719 | 1.720 |
Rmerge | 0.047 | 0.561 |
Number of reflections | 26060 | |
<I/σ(I)> | 23.6 | 2.32 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M sodium citrate tribasic diehydrate, 0.1M HEPES sodium, 20% v/v 2-propanol |