4ZFR
Catalytic domain of Sst2 F403A mutant bound to ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.139, 58.174, 56.442 |
| Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
| Resolution | 53.270 - 1.720 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k1r |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.896 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.270 | 1.750 |
| High resolution limit [Å] | 1.719 | 1.720 |
| Rmerge | 0.047 | 0.561 |
| Number of reflections | 26060 | |
| <I/σ(I)> | 23.6 | 2.32 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M sodium citrate tribasic diehydrate, 0.1M HEPES sodium, 20% v/v 2-propanol |
