4ZFO
J22.9-xi: chimeric mouse/human antibody against human BCMA (CD269)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.801, 110.139, 137.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.258 - 1.895 |
| R-factor | 0.1779 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eo9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.202 |
| Data reduction software | XDS (July 4, 2012) |
| Phasing software | PHASER (CCP4Interface 2.1.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.268 | 2.010 |
| High resolution limit [Å] | 1.895 | 1.900 |
| Rmerge | 0.180 | |
| Number of reflections | 87925 | |
| <I/σ(I)> | 10.46 | 1.76 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 1 microliter of the complex at 8 mg/ml with 2 microliters of 21% PEG 3350, 0.1 M BisTris pH 6.5 and 5 mM CuCl2 |
