4ZFH
Crystal structure of Artificial ankyrin repeat protein_Ank(GAG)1D4 mutant -Y56A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.988 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.839, 28.638, 53.537 |
| Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| R-factor | 0.21298 |
| Rwork | 0.210 |
| R-free | 0.27488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.898 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.890 |
| Number of reflections | 11686 |
| <I/σ(I)> | 6 |
| Completeness [%] | 98.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291.15 | PEG 1000, reagent alcohol, sodium-phosphate dibasic/citric acid |
