4ZFH
Crystal structure of Artificial ankyrin repeat protein_Ank(GAG)1D4 mutant -Y56A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.988 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.839, 28.638, 53.537 |
Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.890 |
R-factor | 0.21298 |
Rwork | 0.210 |
R-free | 0.27488 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.890 |
Number of reflections | 11686 |
<I/σ(I)> | 6 |
Completeness [%] | 98.0 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291.15 | PEG 1000, reagent alcohol, sodium-phosphate dibasic/citric acid |