4ZEX
Crystal structure of PfHAD1 in complex with glyceraldehyde-3-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-16 |
Detector | NOIR-1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.600, 44.600, 84.100 |
Unit cell angles | 90.00, 101.30, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.000 |
R-factor | 0.176 |
Rwork | 0.173 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qjb |
RMSD bond length | 0.007 |
RMSD bond angle | 0.969 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 38024 | |
<I/σ(I)> | 13.78 | 2.2 |
Completeness [%] | 98.4 | 97.7 |
Redundancy | 3.79 | 3.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES, pH 7.5, 20% PEG8000 |