4ZET
Blood dendritic cell antigen 2 (BDCA-2) complexed with GalGlcNAcMan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 37.559, 67.645, 118.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.560 - 2.900 |
R-factor | 0.182 |
Rwork | 0.178 |
R-free | 0.26300 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1760) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.560 | 37.560 | 3.070 |
High resolution limit [Å] | 2.900 | 8.690 | 2.900 |
Rmerge | 0.146 | 0.092 | 0.510 |
Rpim | 0.074 | 0.048 | 0.242 |
Total number of observations | 37251 | 1415 | 5646 |
Number of reflections | 7094 | ||
<I/σ(I)> | 8.7 | 13.7 | 4.3 |
Completeness [%] | 99.2 | 98.1 | 97.4 |
Redundancy | 5.3 | 4.6 | 5.2 |
CC(1/2) | 0.985 | 0.985 | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | protein solution comprising 6.2 mg/ml BDCA-2, 5 mM CaCl2, 10 mM Tris-Cl, pH 8.0, 25 mM NaCl and 20 mM GalGlcNAcMan. The reservoir solution contained 0.2 NH4Cl and 20% polyethylene glycol 3.35K |