4ZEP
Structure of Gan1D, a 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with 6-phospho-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.158, 68.944, 152.896 |
Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
Resolution | 28.310 - 2.210 |
R-factor | 0.15354 |
Rwork | 0.151 |
R-free | 0.20703 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ze4 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.799 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.301 | |
Number of reflections | 55496 | |
<I/σ(I)> | 11.4 | 3.3 |
Completeness [%] | 99.5 | 94.7 |
Redundancy | 3.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-18% PEG 8K, 3% MPD, 0.1M imidazole buffer pH 6.5 |