4ZEP
Structure of Gan1D, a 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with 6-phospho-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.158, 68.944, 152.896 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 28.310 - 2.210 |
| R-factor | 0.15354 |
| Rwork | 0.151 |
| R-free | 0.20703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ze4 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.799 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.301 | |
| Number of reflections | 55496 | |
| <I/σ(I)> | 11.4 | 3.3 |
| Completeness [%] | 99.5 | 94.7 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-18% PEG 8K, 3% MPD, 0.1M imidazole buffer pH 6.5 |
