4ZEN
Structure of Gan1D, a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with 6-phospho-beta-galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-01 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.517, 68.674, 153.281 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 26.220 - 1.930 |
| R-factor | 0.15903 |
| Rwork | 0.157 |
| R-free | 0.20187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZEH |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.316 | |
| Number of reflections | 83233 | |
| <I/σ(I)> | 16.5 | 2.1 |
| Completeness [%] | 97.1 | 79.1 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-18% PEG 8K, 0.1 M imidazole pH 6.5, 3% MPD |
