4ZEF

Crystal structure of substrate binding domain 2 (SBD2) OF ABC transporter GLNPQ from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-31
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	81.140, 81.140, 102.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.294 - 1.400
R-factor	0.1301
Rwork	0.129
R-free	0.15840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kqp
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.400	1.400
Rmerge	0.047
Number of reflections	67836
<I/σ(I)>	23.6	1.26
Completeness [%]	100.0	100
Redundancy	12.8	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.2 M potassium sodium tartrate, 0.1 M Na citrate, 2.0 M ammonium sulfate