4ZEE
X-ray structure of the bis-platinum lysozyme adduct formed in the reaction between the protein and the two drugs Cisplatin and Oxaliplatin (preparation 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-27 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.377, 77.377, 37.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.710 - 1.950 |
R-factor | 0.16223 |
Rwork | 0.159 |
R-free | 0.22986 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j1a |
RMSD bond length | 0.020 |
RMSD bond angle | 4.931 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.710 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.113 | 0.611 |
Number of reflections | 8452 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 95.8 | 83.2 |
Redundancy | 5.7 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | Best crystals grow within 2-10 days from the following conditions: 0.6 M NaNO3, 0.1 M sodium acetate pH 4.4 and 20 % ethylene glycol. |