4ZDV
Crystal structure of LC3 in complex with FAM134B LIR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-03-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 62.170, 62.170, 74.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.709 - 1.800 |
R-factor | 0.1861 |
Rwork | 0.184 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eci |
RMSD bond length | 0.007 |
RMSD bond angle | 1.069 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.170 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.527 |
Number of reflections | 14123 | |
<I/σ(I)> | 17.1 | |
Completeness [%] | 100.0 | |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8% Tacsimate (Hampton Research) pH 8.0, 20% Poly ethylene glycol 3350 |