4ZDU

Crystal structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.10
Spacegroup name	H 3 2
Unit cell lengths	110.250, 110.250, 204.361
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.826 - 2.300
R-factor	0.191
Rwork	0.188
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ul1
RMSD bond length	0.003
RMSD bond angle	0.808
Data reduction software	Aimless
Data scaling software	Aimless (0.3.11)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.830	31.830	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.099	0.030	0.802
Rpim	0.034	0.010	0.274
Total number of observations	197452	3560	19667
Number of reflections	21538
<I/σ(I)>	14.4	40.9	3.3
Completeness [%]	100.0	97.9	100
Redundancy	9.2	8.7	9.5
CC(1/2)	0.999	0.999	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 4000