4ZDT
Crystal structure of the RING finger domain of Slx1 in complex with the C-terminal domain of Slx4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28238 |
| Spacegroup name | P 41 |
| Unit cell lengths | 85.233, 85.233, 74.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.100 - 2.000 |
| R-factor | 0.21617 |
| Rwork | 0.215 |
| R-free | 0.23631 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.139 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.483 |
| Number of reflections | 36121 | |
| <I/σ(I)> | 28.9 | 7.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.9 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 1.34M ammonium sulfate, 0.1M Bis-Tris, pH 6.8 |
