4ZDS
Crystal Structure of core DNA binding domain of Arabidopsis Thaliana Transcription Factor Ethylene-Insensitive 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.022, 45.174, 47.571 |
| Unit cell angles | 89.46, 77.07, 71.38 |
Refinement procedure
| Resolution | 46.260 - 1.780 |
| R-factor | 0.17652 |
| Rwork | 0.174 |
| R-free | 0.21806 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.126 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.260 |
| High resolution limit [Å] | 1.780 |
| Number of reflections | 23591 |
| <I/σ(I)> | 22.81 |
| Completeness [%] | 96.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 25% w/v Polyethylene glycol 3350 |
