4ZDM
Pleurobrachia bachei iGluR3 LBD Glycine Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-18 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 159.850, 159.850, 55.614 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.384 - 1.500 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.17690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yki |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (dev_1951) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.039 | 0.940 |
| Number of reflections | 67078 | |
| <I/σ(I)> | 59.4 | 2.5 |
| Completeness [%] | 98.7 | 97.6 |
| Redundancy | 10.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Reservoir: 10% PEG 400, 1.6 M Li2SO4, 200 mM MgSO4, 100 mM cacodylate. Protein buffer: 100 mM NaCl, 10 mM HEPES, pH 7.5, 0.5 mM EDTA, 2 mM glutamate |
