4ZDH
Crystal structure of JKA6 TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.695, 60.087, 82.462 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 19.490 - 2.098 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.22350 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.490 | 2.180 |
| High resolution limit [Å] | 2.098 | 2.098 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 25704 | |
| <I/σ(I)> | 3.4 | 18.5 |
| Completeness [%] | 87.9 | 84.3 |
| Redundancy | 3.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2 M LITHIUM SULFATE, 0.1 M TRIS-HCL, PEG 4000 |
