4ZDG

Structure of the Adenovirus 14p1 knob domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	P 63
Unit cell lengths	112.414, 112.414, 79.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.680 - 3.200
R-factor	0.2017
Rwork	0.200
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f0y
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.680	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.179	1.040
<I/σ(I)>		1.6
Completeness [%]		98.4
Redundancy	6.8	6.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	1.65 M MgSO4