4ZDF
Crystal structure of yeast enoyl-CoA isomerase helix-10 deletion (ScECI2-H10) mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2014-02-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 118.121, 118.121, 87.417 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.940 - 1.810 |
R-factor | 0.16062 |
Rwork | 0.159 |
R-free | 0.18785 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k39 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.464 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.940 | 1.870 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.073 | 1.064 |
Number of reflections | 64106 | |
<I/σ(I)> | 17.3 | 1.6 |
Completeness [%] | 99.7 | 97.2 |
Redundancy | 10.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 100 mM imidazole pH 6.5, 1 M NaAcetate |