4ZCL
Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.430, 160.010, 90.020 |
Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
Resolution | 48.181 - 3.060 |
R-factor | 0.2342 |
Rwork | 0.232 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zci |
RMSD bond length | 0.003 |
RMSD bond angle | 0.741 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (dev_1819) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.181 | 3.250 |
High resolution limit [Å] | 3.060 | 3.060 |
Rmerge | 0.100 | 0.680 |
Number of reflections | 29871 | |
<I/σ(I)> | 8.6 | 2 |
Completeness [%] | 99.3 | 98.8 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl, 2 % PEG 6000, and 5 mM [Co(NH3)6]Cl3, pH 8.0 |