4ZCK
Crystal Structure of C-terminal Fragment of Escherichia coli BipA/TypA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.562, 83.562, 191.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 2.480 |
R-factor | 0.1961 |
Rwork | 0.194 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e3x |
RMSD bond length | 0.010 |
RMSD bond angle | 1.286 |
Data reduction software | MOSFLM |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1819) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.580 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.140 | 1.300 |
Number of reflections | 24915 | |
<I/σ(I)> | 6.5 | 1 |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 7.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 3.04 M sodium formate, 200 mM Tris-HCl, and 1 mM magnesium bromide, pH 7.6 |