4ZCI

Crystal Structure of Escherichia coli GTPase BipA/TypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	56.717, 161.391, 89.601
Unit cell angles	90.00, 97.95, 90.00

Refinement procedure

Resolution	46.004 - 2.627
R-factor	0.2123
Rwork	0.210
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cb4 3e3x
RMSD bond length	0.003
RMSD bond angle	0.744
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1819)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.004	2.680
High resolution limit [Å]	2.627	2.630
Rmerge	0.106	0.919
Number of reflections	46760
<I/σ(I)>	12.4	1.4
Completeness [%]	99.7	96.1
Redundancy	3.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM Tris-HCl, 2 % PEG 6000, and 5 mM [Co(NH3)6]Cl3, pH 8.0