4ZCE
Crystal Structure of the dust mite allergen Der p 23 from Dermatophagoides pteronyssinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-04-09 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.283, 53.519, 40.395 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 26.759 - 1.550 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.19030 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 15162 | |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 96.9 | |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.5 | 277 | 50% PBS buffer for two months in |
