4ZCD
Renalase in complex with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97895 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.700, 37.600, 138.200 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 33.195 - 1.661 |
| R-factor | 0.1518 |
| Rwork | 0.151 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 (2.3.10) |
| Data scaling software | HKL-2000 (2.3.10) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.044 | 0.336 |
| Number of reflections | 76829 | |
| <I/σ(I)> | 26.3 | 3.7 |
| Completeness [%] | 99.7 | 94 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2.0 M Sodium formate, 100 mM sodium acetate, pH 4.6 |
