4ZCC
Renalase in complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.500, 71.500, 143.800 |
| Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
| Resolution | 46.224 - 1.997 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.634 |
| Data reduction software | HKL-2000 (2.3.10) |
| Data scaling software | HKL-2000 (2.3.10) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.224 | 2.030 |
| High resolution limit [Å] | 1.997 | 1.997 |
| Rmerge | 0.096 | 0.628 |
| Number of reflections | 86594 | |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2.0 M Sodium formate, 100 mM sodium acetate, pH 4.6 |
