4ZCB
Human CRBPII mutant - Y60W dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.620, 109.774, 36.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.590 - 1.700 |
| R-factor | 0.18487 |
| Rwork | 0.182 |
| R-free | 0.23884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qyn |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.112 |
| Data reduction software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 26934 | |
| <I/σ(I)> | 66.13 | 7.71 |
| Completeness [%] | 98.6 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 30% PEG 4000, 0.1M CH3COONa.3H2O, pH 4.6, 0.1M CH3COONH4 |
