4ZCA
Eg5 motor domain mutant Y231F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-07 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.540, 77.830, 93.120 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| Rwork | 0.246 |
| R-free | 0.28920 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (Proteum2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.116 | 0.375 |
| Number of reflections | 32744 | |
| <I/σ(I)> | 8.49 | 1.91 |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 4.54 | 2.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 200 mM NaNO3, 20-25% PEG 3350, 100 mM MES pH 5.5-5.7, 5-10% glycerol |
