4ZC4
Crystal structure of LARP1-unique domain DM15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-04-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.906, 61.759, 85.336 |
| Unit cell angles | 90.00, 116.20, 90.00 |
Refinement procedure
| Resolution | 38.285 - 1.860 |
| R-factor | 0.1822 |
| Rwork | 0.181 |
| R-free | 0.21370 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 (2.3.2) |
| Data scaling software | HKL-2000 (2.3.2) |
| Phasing software | PHENIX (1.8.2) |
| Refinement software | PHENIX (dev_2006) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.570 | |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.153 | 0.108 |
| Number of reflections | 87993 | |
| <I/σ(I)> | 34.582 | 1.4 |
| Completeness [%] | 95.0 | 63.66 |
| Redundancy | 5.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 304 | 0.225 M ammonium chloride, pH 6.3, 23% PEG 3350 |
