4ZBZ

Family 4 uracil-DNA glycosylase from Sulfolobus tokodaii (free form, X-ray wavelength=1.5418)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	52.030, 52.345, 74.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.172 - 1.900
R-factor	0.1626
Rwork	0.160
R-free	0.20620
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4zbx
RMSD bond length	0.009
RMSD bond angle	1.126
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.059	0.325
Number of reflections	16590
<I/σ(I)>	25.3	5.2
Completeness [%]	99.7	97.5
Redundancy	8.1	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	20% PEG3350, 0.1 M MES pH5.8, 20% glycerol