4ZBY
Family 4 uracil-DNA glycosylase from Sulfolobus tokodaii (uracil complex form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-28 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.056, 52.222, 74.062 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.679 - 1.700 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.20650 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4zbx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.397 |
| Number of reflections | 23005 | |
| <I/σ(I)> | 37.6 | 4.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 15% PEG3350, 0.1 M MES |
