4ZBY
Family 4 uracil-DNA glycosylase from Sulfolobus tokodaii (uracil complex form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-09-28 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.056, 52.222, 74.062 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.679 - 1.700 |
R-factor | 0.1669 |
Rwork | 0.165 |
R-free | 0.20650 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4zbx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.195 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.056 | 0.397 |
Number of reflections | 23005 | |
<I/σ(I)> | 37.6 | 4.5 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 5.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 15% PEG3350, 0.1 M MES |