4ZBC
A dehydrated form of glucose isomerase collected at 100K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.629, 93.641, 97.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.580 - 2.000 |
| R-factor | 0.13778 |
| Rwork | 0.136 |
| R-free | 0.18196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mnz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.029 |
| Data reduction software | xia2 (xia2/dials) |
| Data scaling software | xia2 (xia2/dials) |
| Phasing software | MOLREP (11/3.01) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.580 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.103 |
| Number of reflections | 50965 | |
| <I/σ(I)> | 14.4 | 8.6 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Hepes, 10% PEG 400, 0.05 M Manganese Chloride, 20% Glucose |
