4ZBA
Crystal structure of the glutathione transferase URE2P8 from Phanerochaete chrysosporium with oxidized glutathione.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.641, 91.872, 180.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.300 - 1.501 |
| R-factor | 0.1507 |
| Rwork | 0.150 |
| R-free | 0.16890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f0c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.044 | 0.232 |
| Number of reflections | 136502 | |
| <I/σ(I)> | 25.8 | 4.9 |
| Completeness [%] | 95.9 | 77.1 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 278 | 30 % PEG4000, 0.1M TRIS pH8.5, 0.2M Sodium Acetate trihydrate |
