4ZB8
Crystal structure of the glutathione transferase URE2P6 from Phanerochaete chrysosporium in complex with oxidized glutathione.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.848, 77.378, 89.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.088 - 2.000 |
R-factor | 0.1924 |
Rwork | 0.192 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zba |
RMSD bond length | 0.008 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.168 | 0.609 |
Number of reflections | 14950 | |
<I/σ(I)> | 16.9 | 8.2 |
Completeness [%] | 87.8 | 97.7 |
Redundancy | 7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 278 | 20 % PEG10000 0.1M HEPES pH7.5 |