4ZB6
Crystal structure of glutathione transferase URE2P4 from Phanerochaete chrysosporium in complex with oxidized glutathione.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.437, 94.059, 104.627 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.000 - 1.801 |
R-factor | 0.1647 |
Rwork | 0.163 |
R-free | 0.19040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zb8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.893 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.761 | |
Number of reflections | 84906 | |
<I/σ(I)> | 14.5 | 3.3 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 7.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 278 | 30 % PEG4000, 0.1M Sodium Acetate pH4.6, 0.2M Ammonium Acetate |