4ZB4
Spliceosome component
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.220, 100.140, 65.340 |
| Unit cell angles | 90.00, 92.33, 90.00 |
Refinement procedure
| Resolution | 48.992 - 2.300 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lrv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.396 |
| High resolution limit [Å] | 2.300 | 2.331 |
| Rmerge | 0.490 | |
| Number of reflections | 31956 | |
| <I/σ(I)> | 12.35 | |
| Completeness [%] | 99.3 | |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | PEG 4000 |
