4ZAZ
Structure of UbiX Y169F in complex with a covalent adduct formed between reduced FMN and dimethylallyl monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | F 2 3 |
Unit cell lengths | 142.260, 142.260, 142.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.300 - 1.450 |
R-factor | 0.10469 |
Rwork | 0.103 |
R-free | 0.14723 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.025 |
RMSD bond angle | 2.228 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.300 | 1.490 |
High resolution limit [Å] | 1.450 | 1.450 |
Rpim | 0.028 | 0.356 |
Number of reflections | 40068 | |
<I/σ(I)> | 15.7 | 2.4 |
Completeness [%] | 99.9 | |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 12% PEG 3350, 150mM sodium thiocyanate, and 100mM Tris pH 7.2 |