4ZAX
Structure of UbiX in complex with oxidised prenylated FMN (radical)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 141.790, 141.790, 141.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.750 - 1.610 |
| R-factor | 0.14493 |
| Rwork | 0.144 |
| R-free | 0.17095 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.431 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.750 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.024 | 0.335 |
| Number of reflections | 29027 | |
| <I/σ(I)> | 20.1 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 12% PEG 3350, 150mM sodium thiocyanate and 100mM Tris pH 7.2 |
