4ZAW
Structure of UbiX in complex with reduced prenylated FMN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-24 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | F 2 3 |
Unit cell lengths | 142.740, 142.740, 142.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.040 - 1.890 |
R-factor | 0.14969 |
Rwork | 0.148 |
R-free | 0.19160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.972 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.040 | 1.940 |
High resolution limit [Å] | 1.890 | 1.890 |
Rpim | 0.043 | 0.330 |
Number of reflections | 18350 | |
<I/σ(I)> | 13.4 | 2.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 12% PEG 3350, 150mM sodium thiocyanate, and 100mM Tris pH 7.2 |