4ZAV
UbiX in complex with a covalent adduct between dimethylallyl monophosphate and reduced FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 142.180, 142.180, 142.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.270 - 1.400 |
| R-factor | 0.09927 |
| Rwork | 0.098 |
| R-free | 0.12900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.983 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.270 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rpim | 0.027 | 0.297 |
| Number of reflections | 44084 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 12% PEG 3350, 150mM sodium thiocyanate, and 100mM Tris pH 7.2 |
