4ZAJ

2.2 Angstrom Crystal Structure of a Human Arginyl-tRNA Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-03-26
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	54.720, 61.920, 208.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.160 - 2.220
R-factor	0.2211
Rwork	0.219
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q2t
RMSD bond length	0.009
RMSD bond angle	1.318
Data reduction software	xia2
Data scaling software	xia2 (0.3.8.0\)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.160	38.160	2.280
High resolution limit [Å]	2.220	9.930	2.220
Rmerge	0.130	0.049	0.681
Rpim	0.053	0.021	0.294
Total number of observations	270841	2717	18079
Number of reflections	35995
<I/σ(I)>	12.2	28	2.9
Completeness [%]	100.0	97.4	100
Redundancy	7.5	5.7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG 1000