4Z9W
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd)-Native-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.320, 120.200, 44.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.180 - 1.770 |
| R-factor | 0.18129 |
| Rwork | 0.180 |
| R-free | 0.21207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.563 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.320 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.430 | |
| Number of reflections | 73778 | |
| <I/σ(I)> | 10.1 | 4 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 6.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1 M HEPES, 20% w/v PEG 10000, No-cryo protectant used |
