4Z9Q
Crystal structure of OXA-58 with disordered active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.360, 39.790, 72.090 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 37.380 - 2.230 |
| R-factor | 0.1951 |
| Rwork | 0.192 |
| R-free | 0.25259 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.315 |
| Data reduction software | SCALA |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.380 | 2.314 |
| High resolution limit [Å] | 2.230 | 2.235 |
| Rmerge | 0.030 | 0.150 |
| Number of reflections | 10289 | |
| <I/σ(I)> | 16.39 | 5.84 |
| Completeness [%] | 98.3 | 93.1 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 1500 |
