4Z9P

Crystal structure of Ebola virus nucleoprotein core domain at 1.8A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97848
Spacegroup name	P 21 21 2
Unit cell lengths	59.845, 162.877, 31.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.719 - 1.792
R-factor	0.1923
Rwork	0.191
R-free	0.22510
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.978
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.792	4.880	1.800
Rmerge	0.084	0.064	0.546
Rmeas	0.088	0.068	0.575
Rpim	0.027	0.022	0.179
Total number of observations	312835
Number of reflections	29588
<I/σ(I)>	6.7
Completeness [%]	99.8	99.8	99.9
Redundancy	10.6	9.9	10
CC(1/2)		0.989	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	289	200mM ammonium citrate tribasic pH 7.0, 20% (w/v) PEG 3350